Zachariae W, Shevchenko A, Andrews P D, Ciosk R, Galova M, Stark M J, Mann M, Nasmyth K
Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.
Science. 1998 Feb 20;279(5354):1216-9. doi: 10.1126/science.279.5354.1216.
Entry into anaphase and exit from mitosis depend on a ubiquitin-protein ligase complex called the anaphase-promoting complex (APC) or cyclosome. At least 12 different subunits were detected in the purified particle from budding yeast, including the previously identified proteins Apc1p, Cdc16p, Cdc23p, Cdc26p, and Cdc27p. Five additional subunits purified in low nanogram amounts were identified by tandem mass spectrometric sequencing. Apc2p, Apc5p, and the RING-finger protein Apc11p are conserved from yeast to humans. Apc2p is similar to the cullin Cdc53p, which is a subunit of the ubiquitin-protein ligase complex SCFCdc4 required for the initiation of DNA replication.
进入后期和退出有丝分裂依赖于一种名为后期促进复合物（APC）或细胞周期体的泛素-蛋白质连接酶复合物。在从芽殖酵母纯化的颗粒中检测到至少12种不同的亚基，包括先前鉴定的蛋白质Apc1p、Cdc16p、Cdc23p、Cdc26p和Cdc27p。通过串联质谱测序鉴定出另外5种以低纳克量纯化的亚基。Apc2p、Apc5p和具有环指结构的蛋白质Apc11p从酵母到人类都是保守的。Apc2p类似于cullin Cdc53p，后者是DNA复制起始所需的泛素-蛋白质连接酶复合物SCFCdc4的一个亚基。
