Chryssanthou E, Grönfors C, Khanna N
Department of Laboratory Medicine, Karolinska Institute & Hospital, Stockholm, Sweden.
Mycoses. 1997 Dec;40(11-12):423-7. doi: 10.1111/j.1439-0507.1997.tb00179.x.
Forty Cryptococcus neoformans strains isolated from cerebral spinal fluid specimens collected from 39 patients were included in the study. The MICs for fluconacole were determined by YNB macrodilution test, microdilution tests using both RPMI1640 and YNB medium and E-tests on solidified RPMI1640 medium, Casitone and YNB agar. In comparison with the reference macrodilution method NCCLS M27-P both the microdilution as well as the E-test techniques can be used for fluconacole susceptibility testing of Cr. neoformans.
从39名患者的脑脊液标本中分离出的40株新型隐球菌菌株被纳入该研究。通过YNB大量稀释试验、使用RPMI1640和YNB培养基的微量稀释试验以及在凝固的RPMI1640培养基、酪蛋白胰消化物和YNB琼脂上进行E试验来测定氟康唑的最低抑菌浓度（MIC）。与参考大量稀释方法NCCLS M27 - P相比，微量稀释法以及E试验技术均可用于新型隐球菌的氟康唑药敏试验。
