Pfaller M A, Messer S A, Boyken L, Rice C, Tendolkar S, Hollis R J, Diekema D J
Department of Pathology, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA.
J Clin Microbiol. 2004 Jan;42(1):380-3. doi: 10.1128/JCM.42.1.380-383.2004.
We evaluated the NCCLS M44-P fluconazole disk diffusion method in comparison with the NCCLS M27-A2 broth microdilution method for determining the susceptibility of 276 isolates of Cryptococcus neoformans. Disk diffusion testing was performed using Mueller-Hinton agar supplemented with 2% glucose and 0.5 microg of methylene blue/ml. Among the 276 isolates, 259 (93.8%) were susceptible, 16 (5.8%) were susceptible--dose dependent, and 1 (0.4%) was resistant to fluconazole as determined by the NCCLS broth microdilution method. The overall categorical agreement between the two methods was 86%, with 0% very major errors, 2% major errors, and 12% minor errors. The disk diffusion method using Mueller-Hinton agar supplemented with glucose and methylene blue appears to be a useful approach for determining the fluconazole susceptibility of C. neoformans.
我们评估了NCCLS M44-P氟康唑纸片扩散法，并与NCCLS M27-A2肉汤微量稀释法进行比较，以确定276株新生隐球菌的药敏情况。纸片扩散试验采用添加2%葡萄糖和0.5微克/毫升亚甲蓝的Mueller-Hinton琼脂进行。根据NCCLS肉汤微量稀释法测定，在276株分离株中，259株（93.8%）对氟康唑敏感，16株（5.8%）为剂量依赖性敏感，1株（0.4%）对氟康唑耐药。两种方法的总体分类一致性为86%，其中极重大错误为0%，重大错误为2%，微小错误为12%。使用添加葡萄糖和亚甲蓝的Mueller-Hinton琼脂的纸片扩散法似乎是一种确定新生隐球菌对氟康唑药敏情况的有用方法。
