Stockert J C
Z Naturforsch C Biosci. 1979 Dec;34(12):1285-6. doi: 10.1515/znc-1979-1238.
The staining reaction of chromatin by aluminium-hematoxylin was investigated. Intense staining, which proved dependent on nucleic acid content, was achieved by using either the preformed lake, mordanting followed by hematoxylin, hematoxylin alone or the lake at high ionic strength. Extraction procedures removed aluminium ions from chromatin but the lake was retained. Chromatin fluorescence induced by ethidium bromide and acridine orange was abolished after staining with the lake. Intercalating-like forces are suggested for aluminium-hematoxylin binding to chromatin.
研究了苏木精铝对染色质的染色反应。通过使用预制色淀、媒染后用苏木精、单独使用苏木精或在高离子强度下使用色淀，均可实现强烈染色，且这种染色反应依赖于核酸含量。提取程序可从染色质中去除铝离子，但色淀仍保留。用色淀染色后，溴化乙锭和吖啶橙诱导的染色质荧光消失。推测苏木精铝与染色质结合存在类似嵌入的作用力。
