一种辅助因子TIP30特异性增强HIV-1反式激活因子（Tat）激活的转录。
A cofactor, TIP30, specifically enhances HIV-1 Tat-activated transcription.
作者信息
Xiao H, Tao Y, Greenblatt J, Roeder R G
机构信息
Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10021, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2146-51. doi: 10.1073/pnas.95.5.2146.
Abstract
Replication of HIV-1 requires the viral Tat protein, which increases the extent of transcription elongation by RNA polymerase II after activation at the single viral long terminal repeat (LTR) promoter. This effect of Tat on transcription requires Tat interactions with a 5' region (TAR) in nascent transcripts as well as Tat-specific cofactors. The present study identifies a cellular protein, TIP30, that interacts with Tat and with an SRB-containing RNA polymerase II complex both in vivo and in vitro. Coexpression of TIP30 specifically enhances transactivation by Tat in transfected cells, and immunodepletion of TIP30 from nuclear extracts abolishes Tat-activated transcription without affecting Tat-independent transcription. These results implicate TIP30 as a specific coactivator that may enhance formation of a Tat-RNA polymerase II holoenzyme complex.
摘要
HIV-1的复制需要病毒Tat蛋白，该蛋白在单个病毒长末端重复序列（LTR）启动子激活后，可增加RNA聚合酶II的转录延伸程度。Tat对转录的这种作用需要Tat与新生转录本中的5'区域（TAR）以及Tat特异性辅因子相互作用。本研究鉴定出一种细胞蛋白TIP30，它在体内和体外均能与Tat以及含SRB的RNA聚合酶II复合物相互作用。在转染细胞中，TIP30的共表达特异性增强了Tat的反式激活作用，并且从核提取物中免疫去除TIP30会消除Tat激活的转录，而不影响非Tat依赖性转录。这些结果表明TIP30是一种特异性共激活因子，可能会增强Tat-RNA聚合酶II全酶复合物的形成。
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TAK, an HIV Tat-associated kinase, is a member of the cyclin-dependent family of protein kinases and is induced by activation of peripheral blood lymphocytes and differentiation of promonocytic cell lines.TAK是一种与HIV反式激活因子（Tat）相关的激酶，属于细胞周期蛋白依赖性蛋白激酶家族成员，可通过外周血淋巴细胞激活和原单核细胞系分化诱导产生。
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The HIV transactivator TAT binds to the CDK-activating kinase and activates the phosphorylation of the carboxy-terminal domain of RNA polymerase II.HIV反式激活因子TAT与细胞周期蛋白依赖性激酶激活激酶结合，并激活RNA聚合酶II羧基末端结构域的磷酸化。
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The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme.人类免疫缺陷病毒反式激活因子Tat与RNA聚合酶II全酶相互作用。
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