Liu X, Miller M J, Joshi M S, Thomas D D, Lancaster J R
Department of Pediatrics, Louisiana State University Medical Center, 1901 Perdido Street, New Orleans, LA 70112, USA.
Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2175-9. doi: 10.1073/pnas.95.5.2175.
We demonstrate herein dramatic acceleration of aqueous nitric oxide (NO) reaction with O2 within the hydrophobic region of either phospholipid or biological membranes or detergent micelles and demonstrate that the presence of a distinct hydrophobic phase is required. Per unit volume, at low amounts of hydrophobic phase, the reaction of NO with O2 within the membranes is approximately 300 times more rapid than in the surrounding aqueous medium. In tissue, even though the membrane represents only 3% of the total volume, we calculate that 90% of NO reaction with O2 will occur there. We conclude that biological membranes and other tissue hydrophobic compartments are important sites for disappearance of NO and for formation of NO-derived reactive species and that attenuation of these potentially damaging reactions is an important protective action of lipid-soluble antioxidants such as vitamin E.
我们在此证明，在磷脂或生物膜或去污剂胶束的疏水区域内，一氧化氮（NO）与O2的水相反应显著加速，并证明需要存在独特的疏水相。每单位体积，在少量疏水相的情况下，膜内NO与O2的反应速度比周围水相介质中快约300倍。在组织中，尽管膜仅占总体积的3%，但我们计算出90%的NO与O2的反应将在那里发生。我们得出结论，生物膜和其他组织疏水隔室是NO消失和形成NO衍生活性物种的重要部位，而减弱这些潜在的损伤反应是维生素E等脂溶性抗氧化剂的重要保护作用。
