Hwang Y S, Karrer E E, Thomas B R, Chen L, Rodriguez R L
Section of Molecular and Cellular Biology, University of California, Davis 95616, USA.
Plant Mol Biol. 1998 Feb;36(3):331-41. doi: 10.1023/a:1005956104636.
Expression of alpha-amylase genes during seedling development plays a key role in production of sugar from the starch stored in the cereal seed. Rice alpha-amylase Amy3D promoter/GUS constructs in transgenic rice cell lines were studied to identify cis elements in the promoter of this metabolite-regulated gene. Three sequences having the greatest effects on Amy3D gene expression included the amylase element (TATCCAT), the CGACG element, and a G box-related element (CTACGTGGCCA). These promoter cis elements are needed for high-level expression of Amy3D under conditions of sugar starvation. The involvement of G box cis-elements in environmental stress responses suggest a link between the nutrient stress and the environmental stress responses of the plant.
α-淀粉酶基因在幼苗发育过程中的表达在从谷物种子中储存的淀粉产生糖分的过程中起着关键作用。对转基因水稻细胞系中的水稻α-淀粉酶Amy3D启动子/GUS构建体进行了研究，以鉴定这种代谢物调节基因启动子中的顺式元件。对Amy3D基因表达影响最大的三个序列包括淀粉酶元件（TATCCAT）、CGACG元件和一个G盒相关元件（CTACGTGGCCA）。这些启动子顺式元件是在糖饥饿条件下Amy3D高水平表达所必需的。G盒顺式元件参与环境胁迫反应表明植物的营养胁迫与环境胁迫反应之间存在联系。
