Schrenk MO, Edwards KJ, Goodman RM, Hamers RJ, Banfield JF
M. O Schrenk and K. J. Edwards, Department of Geology and Geophysics, University of Wisconsin-Madison, 1215 West Dayton Street, Madison, WI 53706, USA. R. M. Goodman, Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden D.
Science. 1998 Mar 6;279(5356):1519-22. doi: 10.1126/science.279.5356.1519.
Although Thiobacillus ferrooxidans and Leptospirillum ferrooxidans are widely considered to be the microorganisms that control the rate of generation of acid mine drainage, little is known about their natural distribution and abundance. Fluorescence in situ hybridization studies showed that at Iron Mountain, California, T. ferrooxidans occurs in peripheral slime-based communities (at pH over 1.3 and temperature under 30 degreesC) but not in important subsurface acid-forming environments (pH 0.3 to 0.7, temperature 30 degrees to 50 degreesC). Leptospirillum ferrooxidans is abundant in slimes and as a planktonic organism in environments with lower pH. Thiobacillus ferrooxidans affects the precipitation of ferric iron solids but plays a limited role in acid generation, and neither species controls direct catalysis at low pH at this site.
尽管氧化亚铁硫杆菌和氧化亚铁钩端螺旋菌被广泛认为是控制酸性矿山废水产生速率的微生物，但对它们的自然分布和丰度却知之甚少。荧光原位杂交研究表明，在加利福尼亚州的铁山，氧化亚铁硫杆菌存在于外围基于黏液的群落中（pH值超过1.3且温度低于30摄氏度），但不存在于重要的地下产酸环境中（pH值0.3至0.7，温度30摄氏度至50摄氏度）。氧化亚铁钩端螺旋菌在黏液中含量丰富，并且在较低pH值的环境中作为浮游生物存在。氧化亚铁硫杆菌影响三价铁固体的沉淀，但在产酸过程中作用有限，而且在该地点的低pH值条件下，这两个物种都不控制直接催化作用。
