Roesijadi G, Brubacher L L, Unger M E, Anderson R S
University of Maryland, Chesapeake Biological Laboratory, Solomons, MD 20688, USA.
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1997 Oct;118(2):171-6. doi: 10.1016/s0742-8413(97)00085-6.
(1) Hemocytes of the oyster Crassostrea virginica were exposed to Cd in vitro to examine the concomitant induction of metallothionein (MT) mRNA and production of reactive oxygen species (ROS) during the oxidative burst response of phagocytic cells. (2) MT mRNA induction was concentration-dependent, exhibiting a threshold between 5 and 10 microM cdCl2, and an increase at higher Cd concentrations up to a maximum level of 36 microM cdCl2. Continued increases in the Cd exposure concentrations resulted in declines in the levels of MT mRna. (3) No significant effects of Cd on ROS production were observed at Cd concentrations up to 36 microM CdCl2. However, ROS production decreased in a concentration-dependent fashion at CdCl2 concentrations from 36 to 356 microM, the highest concentration tested. (4) At these higher concentrations of Cd, the concomitant decrease in MT mRNA inducibility and suppression of ROS production were probably manifestations of the general cytotoxicity of Cd. ROS suppression can result in reduced resistance to infectious agents, suggesting that Cd is immunotoxic to C. virginica.
