Zuskin E, Mustajbegović J, Sitar-Srebocan V
Skola narodnog zdravlja Andrija Stampar Medicinskog fakulteta Sveucilista u Zagrebu.
Lijec Vjesn. 1997 Mar-Apr;119(3-4):103-5.
The effect of brewery dust extracts was studied on 28 isolated nonsensitized guinea pig tracheal smooth muscle. Dust extracts of barley, hops and brewery yeast were prepared in a concentration of 1:10 w/v. Biological activity (constriction of smooth muscle) of dust extracts was expressed as a percent of initial maximal carbacol contraction 10(-4) M. Pharmacological studies were performed by pretreating guinea pig tracheal tissue with drugs such as atropin (10(-6) M), pirilamin (10(-6) M), indometacin (10(-6) M) i verapamil (10(-5) M). Constrictor effects of the dust extracts was significantly inhibited by atropine suggesting an interaction of the extracts with parasympathetic nerves. Inhibition of contraction of other mediators was less effective and varied with dust extract. Our data suggest that brewery dust extracts cause a dose related airway smooth muscle constriction by nonimmunological mechanisms involving cholinergic receptors and a variety of airway mediators.
研究了啤酒厂粉尘提取物对28条分离的未致敏豚鼠气管平滑肌的作用。制备了浓度为1:10 w/v的大麦、啤酒花和啤酒酵母的粉尘提取物。粉尘提取物的生物活性（平滑肌收缩）以初始最大卡巴胆碱收缩（10⁻⁴ M）的百分比表示。通过用阿托品（10⁻⁶ M）、吡咯明（10⁻⁶ M）、吲哚美辛（10⁻⁶ M）和维拉帕米（10⁻⁵ M）等药物预处理豚鼠气管组织进行药理学研究。阿托品显著抑制了粉尘提取物的收缩作用，表明提取物与副交感神经相互作用。对其他介质收缩的抑制效果较差，且因粉尘提取物而异。我们的数据表明，啤酒厂粉尘提取物通过涉及胆碱能受体和多种气道介质的非免疫机制引起剂量相关的气道平滑肌收缩。
