利用补骨脂素-DNA交联和引物延伸技术对核小体进行体内定位。
In vivo mapping of nucleosomes using psoralen-DNA crosslinking and primer extension.
作者信息
Wellinger R E, Sogo J M
机构信息
Institut fur Zellbiologie, ETH-Hönggerberg, CH-8093 Zurich, Switzerland.
出版信息
Nucleic Acids Res. 1998 Mar 15;26(6):1544-5. doi: 10.1093/nar/26.6.1544.
Abstract
By the use of psoralen crosslinking and primer extension, a method was developed which allows the analysis of chromatin structure in vivo. Using a yeast minichromosome, >9 nucleosomes were mapped with a resolution of at least +/-30 bp.
摘要
通过使用补骨脂素交联和引物延伸技术，开发了一种可用于体内染色质结构分析的方法。利用酵母微型染色体，定位了9个以上的核小体，分辨率至少为±30碱基对。
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