Ye X P, Donnelly C A, Fu Y L, Wu Z X
Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, UK.
Trop Med Int Health. 1997 Mar;2(3):261-4. doi: 10.1046/j.1365-3156.1997.d01-259.x.
Experiments were performed to describe the distribution of Trichuris trichiura and Ascaris lumbricoides eggs in faeces and to test for the effect of stirring faecal specimens prior to sampling. For each of 5 volunteers infected with A. lumbricoides and 7 volunteers infected with T. trichiura, helminth eggs were counted in 150 samples from a single stool specimen using the Kato-Katz smear technique. No significant differences between the distribution of eggs in samples from the surface and the centre of the specimens were found. Prior to stirring, eggs were found to be aggregately distributed in four of the specimens with T. trichiura and all of the specimens with A. lumbricoides. The distributions of eggs were not significantly altered by stirring the stool prior to sampling except in the case of one volunteer infected with A. lumbricoides for whom stirring reduced variability.
进行了实验以描述粪类圆线虫和蛔虫卵在粪便中的分布，并测试在采样前搅拌粪便标本的效果。对于5名感染蛔虫的志愿者和7名感染鞭虫的志愿者，使用加藤厚涂片技术对来自单个粪便标本的150个样本中的蠕虫卵进行计数。未发现标本表面和中心样本中虫卵分布存在显著差异。在搅拌之前，发现4份感染鞭虫的标本和所有感染蛔虫的标本中虫卵呈聚集分布。除了一名感染蛔虫的志愿者，搅拌粪便会降低其变异性外，采样前搅拌粪便对虫卵分布没有显著影响。
