Cai M Y, Arenaz P
Department of Biological Sciences, University of Texas at El Paso 79968, USA.
Mutagenesis. 1998 Jan;13(1):27-32. doi: 10.1093/mutage/13.1.27.
Macrocyclic polyethers (crown ethers) are a family of compounds that possess the ability to complex with and transport metal ions across membranes. Because of their unique ionophoric characteristic, they have wide application in industry and research, chemistry and biology. In the current investigation the relationship between heavy metal mutagenesis and crown ether co-mutagenicity and/or antimutagenicity in mammalian cells has been examined using sister chromatid exchange (SCE) as the cytogenetic end point. Chinese hamster ovary cells were treated with lead or cadmium, with and without selected crown ethers. Several genotoxic end points, including SCEs were scored and statistically compared. We report here that most of the crown ethers studied had little or no influence on lead- or cadmium-induced SCEs or chromosome aberrations. On the other hand, the substituted crown ether dicyclohexyl 21-crown-7 significantly decreased both spontaneous and metal-induced SCE frequencies, suggesting that this crown ether may possess antimutagenic activity.
大环聚醚（冠醚）是一类能够与金属离子络合并跨膜运输金属离子的化合物。由于其独特的离子载体特性，它们在工业、研究、化学和生物学领域有着广泛的应用。在当前的研究中，以姐妹染色单体交换（SCE）作为细胞遗传学终点，研究了哺乳动物细胞中重金属诱变与冠醚共诱变和/或抗诱变之间的关系。用铅或镉处理中国仓鼠卵巢细胞，同时添加或不添加选定的冠醚。对包括SCE在内的几个遗传毒性终点进行了评分并进行统计学比较。我们在此报告，所研究的大多数冠醚对铅或镉诱导的SCE或染色体畸变几乎没有影响。另一方面，取代冠醚二环己基21-冠-7显著降低了自发和金属诱导的SCE频率，表明该冠醚可能具有抗诱变活性。
