Maisel R H, Ogura J H
Ann Otol Rhinol Laryngol. 1976 Jul-Aug;85(4 Pt 1):517-22. doi: 10.1177/000348947608500413.
Dinitrochlorobenzene (DNCB) is a simple chemical useful to evaluate both afferent (sensitizing) and efferent (reacting) limbs of the delayed, cell-mediated immune system. This system invokes the small lymphocyte (T cell) and is apparently part of the host defense against cancer. Severnty-two patients with new primary squamous cell carcinomas of the larynx, hypopharynx and oropharynx were sensitized to DNCB by contact application, and delayed cutaneous hypersensitivity tested. The T cells were also assessed in this study by lymphocyte count. All patients were treated for cure. Ninety-one percent of DNCB reactors were alive and tumor-free at two years. Forty-five percent of negative reactors had recurrent tumor and died of carcinoma. Cases grouped by site among glottic, supraglottic and inferior hypopharynx showed identical results. Lymphocyte counts showed a trend in the same direction. On statistical analysis, all differrences were significant. Eighty-five percent of recurrent tumors occurred in patients who were nonresponders. The present data suggest DNCB reactivity is helpful in predicting outcome after surgery, or combined radiation and surgery, for mucosal squamous carcinoma of the head and neck.
二硝基氯苯（DNCB）是一种简单的化学物质，可用于评估迟发型细胞介导免疫系统的传入（致敏）和传出（反应）环节。该系统涉及小淋巴细胞（T细胞），显然是宿主抗癌防御的一部分。对72例喉、下咽和口咽原发性新鳞状细胞癌患者进行接触性应用DNCB致敏，并检测迟发性皮肤超敏反应。本研究还通过淋巴细胞计数评估T细胞。所有患者均接受根治性治疗。91%的DNCB反应者在两年时存活且无肿瘤。45%的阴性反应者出现肿瘤复发并死于癌症。按声门、声门上和下咽下部部位分组的病例显示出相同的结果。淋巴细胞计数显示出相同方向的趋势。经统计分析，所有差异均具有显著性。85%的复发肿瘤发生在无反应者中。目前的数据表明，DNCB反应性有助于预测头颈部黏膜鳞状癌手术或放疗联合手术治疗后的结果。
