Grizzi I, Braud C, Vert M
C.R.B.A.--URA CNRS 1465, Faculté de Pharmacie, Montpellier, France.
J Biomater Sci Polym Ed. 1998;9(2):189-204. doi: 10.1163/156856298x00514.
In order to analyze the alginate components of alginate dressings and the fractions which are released when the dressing is in contact with model biological fluids, the use of various analytical methods was considered. The first step was the conversion of a calcium alginate batch to pure sodium alginate. The recovery of the latter from either insoluble or soluble mixed sodium/calcium alginates was performed by complexation of calcium ions with sodium citrate followed by ultrafiltration. Comparisons were made between sugar analysis, 1H NMR and circular dichroism (CD) data to determinate the contents in guluronic and mannuronic acids of sodium alginate chains. It was shown that CD measurements afford a rapid and nondestructive method for determination of %G when one takes the ratio theta200/theta220 into account. Fractionation of crude alginate (generally ranging from 30 to 70% G) was achieved by the triangle dissolution/precipitation method in order to increase the range of alginate in sugar composition. The various validated procedures were applied to investigate the effects of irradiation sterilization on alginate dressings. It was shown that sugar composition is retained whereas molecular weight decreased dramatically due to chain scission.
为了分析藻酸盐敷料的藻酸盐成分以及敷料与模拟生物流体接触时释放的组分，考虑使用各种分析方法。第一步是将一批海藻酸钙转化为纯海藻酸钠。通过柠檬酸根离子与钙离子络合，然后进行超滤，从不溶性或可溶性混合钠/钙藻酸盐中回收后者。对糖分析、1H NMR和圆二色性（CD）数据进行了比较，以确定海藻酸钠链中古洛糖醛酸和甘露糖醛酸的含量。结果表明，当考虑θ200/θ220的比值时，CD测量为测定%G提供了一种快速且无损的方法。为了增加藻酸盐在糖组成中的范围，采用三角溶解/沉淀法对粗藻酸盐（通常G含量在30%至70%之间）进行分级分离。应用各种经过验证的程序来研究辐照灭菌对藻酸盐敷料的影响。结果表明，糖组成得以保留，而由于链断裂，分子量显著降低。
