Shah H M, Shah M N, Gokani V N, Jethal B S
Department of Periodontia, Govt. Dental College, Ahmedabad.
Indian J Dent Res. 1993 Jul-Dec;4(3-4):103-11.
A project was launched to evaluate and compare antimicrobial efficacy of Hexidine and Listerine over placebo in 10 day human experimental gingivitis study. A rigid study schedule for subject compliance spanning well over 3 months was sorted out and volunteers recruited for study rinsed all the 3 assigned mouthrinses containing (a) chlorhexidine gluconate, (b) "essential oils" and (c) flavoured distilled water one after the other at certain prefixed intervals. After 10 days of assigned mouthrinse regimen, where the assigned mouthrinse was the only method practiced by the volunteers for oral hygiene, Supragingival Plaque was quantitatively and qualitatively assayed. Qualitatively supragingival plaque was assayed by Gram staining (Direct smear and Thioglycollate Broth) and growth characteristics i.e; Aerobic, Microaerophilic and Anaerobic growth was noted. Quantitatively plaque was assayed by calculating total microbial load per tooth by preparing Mafarland Nephelometer standard and Spectrophotometric analysis. It is concluded that Hexidine and Listerine exert similar antimicrobial efficacy in 10 days experimental gingivitis study. Hexidine and Listerine exert their antimicrobial influence through reduction of total Aerobes and Anaerobes reducing total microbial load per tooth by 58% and 53% respectively as compared to placebo.
在一项为期10天的人体实验性牙龈炎研究中，启动了一个项目来评估和比较洗必泰和李斯德林与安慰剂的抗菌效果。制定了一个严格的研究时间表以确保受试者在超过3个月的时间内遵守规定，招募的志愿者按照特定的固定间隔依次使用三种指定的漱口水，分别含有（a）葡萄糖酸氯己定、（b）“精油”和（c）加味蒸馏水。在进行了10天指定的漱口水使用方案后，此时指定的漱口水是志愿者进行口腔卫生的唯一方法，对龈上菌斑进行了定量和定性分析。通过革兰氏染色（直接涂片和硫乙醇酸盐肉汤）对龈上菌斑进行定性分析，并记录其生长特性，即需氧、微需氧和厌氧生长情况。通过制备马法兰比浊计标准品并进行分光光度分析，计算每颗牙齿的总微生物负荷来对菌斑进行定量分析。得出的结论是，在为期10天的实验性牙龈炎研究中，洗必泰和李斯德林具有相似的抗菌效果。与安慰剂相比，洗必泰和李斯德林通过减少总需氧菌和厌氧菌来发挥抗菌作用，每颗牙齿的总微生物负荷分别降低了58%和53%。
