Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
J Anim Sci Biotechnol. 2013 Mar 8;4(1):10. doi: 10.1186/2049-1891-4-10.
The correlation between IgY levels of the serum and the yolk has been well documented in wild and domestic birds. The levels of total yolk IgY can be an index of the general health status of birds and may contribute to breeding programs when fitness of the offspring is a concern. We measured the levels of total serum IgY and yolk IgY in three different breeds (White Leghorn, Silkie and Dongxiang blue-shell) using indirect ELISA, and found that there was a significantly positive correlation between the levels of total serum IgY and total yolk IgY in all three breeds (White Leghorn: r = 0.404, P < 0.001, n = 100; Silkie: r = 0.561, P < 0.001, n = 70; Dongxiang blue-shell: r = 0.619, P < 0.001, n = 30). We also measured the total serum IgY levels in the 3-day-old offspring hatched from the Silkie hens and results were significantly correlated for serum IgY levels (r = 0.535, P < 0.001, n = 70) and the yolk IgY levels (r = 0.481, P < 0.001, n = 70). The regression analysis showed simple linear regression between IgY levels in hen serum, yolk and offspring serum. Our results suggest that total IgY level could be used as an index for chicken fitness.
血清和蛋黄中 IgY 水平的相关性在野生和家养鸟类中已有很好的记录。总蛋黄 IgY 水平可以作为鸟类一般健康状况的指标，并且当后代的适应性成为关注焦点时，可能有助于繁殖计划。我们使用间接 ELISA 测量了三种不同品种（白来航鸡、丝毛鸡和东乡蓝壳）的血清总 IgY 和蛋黄 IgY 水平，发现所有三种品种的血清总 IgY 和总蛋黄 IgY 水平之间均存在显著正相关（白来航鸡：r=0.404，P<0.001，n=100；丝毛鸡：r=0.561，P<0.001，n=70；东乡蓝壳：r=0.619，P<0.001，n=30）。我们还测量了从丝毛母鸡孵化的 3 日龄雏鸡的血清总 IgY 水平，结果显示血清 IgY 水平（r=0.535，P<0.001，n=70）和蛋黄 IgY 水平（r=0.481，P<0.001，n=70）之间存在显著相关性。回归分析显示母鸡血清、蛋黄和雏鸡血清中 IgY 水平之间存在简单线性回归。我们的结果表明，总 IgY 水平可用作鸡适应性的指标。
