Groth L, Serup J
Department of Dermatological Research, Leo Pharmaceutical Products, Ballerup, Denmark.
Acta Derm Venereol. 1998 Jan;78(1):5-9. doi: 10.1080/00015559850135733.
Cutaneous microdialysis is a method of measuring endogenous and exogenous compounds in the dermal interstitial fluid. The microdialysis probe is inserted in the dermis using a guide cannula. The insertion trauma was studied in dorsal forearm skin in a total of 28 human healthy volunteers. Twenty-four volunteers received local anaesthesia (Xylocain 10 mg/ml) in both forearms and a microdialysis probe was inserted in one of the arms. In 12 volunteers the insertion trauma and the effect of anaesthesia on skin blood flow and erythema were studied by laser Doppler perfusion imaging, Minolta Chromameter CR 200 and Dermaspectrometer. In the other 12 subjects trauma-induced oedema and effects on skin thickness were studied by ultrasound imaging. In addition, a microdialysis probe was inserted without prior anaesthesia in 4 volunteers, and the effects on skin blood flow and erythema were investigated. Significant increases in skin blood flow, erythema and skin thickness were found after insertion of the microdialysis probe. Local anaesthesia prior to the insertion reduced the effects of trauma. Probe depth in dermis did not influence the effects of trauma. At least 90-120 min is required after insertion in order to allow the vascular reaction to needle trauma to return to the baseline range.
皮肤微透析是一种测量真皮间质液中内源性和外源性化合物的方法。使用引导套管将微透析探头插入真皮。在总共28名健康人类志愿者的前臂背部皮肤中研究了插入创伤。24名志愿者双侧前臂接受局部麻醉（10mg/ml利多卡因），并在其中一只手臂插入微透析探头。在12名志愿者中，通过激光多普勒灌注成像、美能达色差仪CR 200和皮肤光谱仪研究了插入创伤以及麻醉对皮肤血流和红斑的影响。在另外12名受试者中，通过超声成像研究了创伤引起的水肿以及对皮肤厚度的影响。此外，4名志愿者未预先麻醉就插入了微透析探头，并研究了其对皮肤血流和红斑的影响。插入微透析探头后发现皮肤血流、红斑和皮肤厚度显著增加。插入前局部麻醉可减轻创伤的影响。探头在真皮中的深度不影响创伤的影响。插入后至少需要90 - 120分钟，以使对针刺创伤的血管反应恢复到基线范围。
