Knuutinen J, Harjula P
Department of Chemistry, University of Jyväskylä, Finland.
J Chromatogr B Biomed Sci Appl. 1998 Jan 23;705(1):11-21. doi: 10.1016/s0378-4347(97)00505-7.
A method for the separation of sarcoplasmic fish proteins by RP-HPLC is described. The procedure revealed significant differences useful for reliable identification of fish species. Sixteen of the most common Finnish freshwater fish species were differentiated by species-specific HPLC chromatograms obtained using photodiode-array detection (PAD) at 200-350 nm. The analytical column was a Hi-Pore RP-304 reversed-phase column. The separation was performed by a linear gradient of acetonitrile and water with a small amount of trifluoracetic acid (TFA). Star-symbol plots were constructed from the chromatograms to visualize the data. Clearly different HPLC protein profiles for most fish species were obtained. The chromatograms of salmonoids show similarities, whereas the protein profiles of cyprinids are dissimilar. Minor intraspecific differences were obtained for three types of powan (Coregonus lavaretus).
本文描述了一种用反相高效液相色谱法（RP-HPLC）分离鱼肉肌浆蛋白的方法。该方法显示出显著差异，有助于可靠鉴定鱼类物种。使用光电二极管阵列检测（PAD）在200-350nm波长下获得的特定物种高效液相色谱图，可区分16种最常见的芬兰淡水鱼物种。分析柱为Hi-Pore RP-304反相柱。采用乙腈和水的线性梯度以及少量三氟乙酸（TFA）进行分离。根据色谱图构建星号图以直观呈现数据。大多数鱼类物种都获得了明显不同的高效液相色谱蛋白质谱。鲑科鱼类的色谱图显示出相似性，而鲤科鱼类的蛋白质谱则不同。对于三种类型的白鲑（Coregonus lavaretus），获得了较小的种内差异。
