对普遍存在的CNC-bZIP转录因子Nrf-1进行靶向破坏，会导致小鼠出现贫血和胚胎致死。
Targeted disruption of the ubiquitous CNC-bZIP transcription factor, Nrf-1, results in anemia and embryonic lethality in mice.
作者信息
Chan J Y, Kwong M, Lu R, Chang J, Wang B, Yen T S, Kan Y W
机构信息
Department of Laboratory Medicine, University of California, San Francisco, CA 94143, USA.
出版信息
EMBO J. 1998 Mar 16;17(6):1779-87. doi: 10.1093/emboj/17.6.1779.
Abstract
The CNC-basic leucine zipper (CNC-bZIP) family is a subfamily of bZIP proteins identified from independent searches for factors that bind the AP-1-like cis-elements in the beta-globin locus control region. Three members, p45-Nf-e2, Nrf-1 and Nrf-2 have been identified in mammals. Expression of p45-Nf-e2 is largely restricted to hematopoietic cells while Nrf-1 and Nrf-2 are expressed in a wide range of tissues. To determine the function of Nrf-1, targeted disruption of the Nrf-1 gene was carried out. Homozygous Nrf-1 mutant mice are anemic due to a non-cell autonomous defect in definitive erythropoiesis and die in utero.
摘要
CNC-碱性亮氨酸拉链（CNC-bZIP）家族是bZIP蛋白的一个亚家族，它是通过独立搜索与β-珠蛋白基因座控制区域中AP-1样顺式元件结合的因子而鉴定出来的。在哺乳动物中已鉴定出三个成员，即p45-Nf-e2、Nrf-1和Nrf-2。p45-Nf-e2的表达主要局限于造血细胞，而Nrf-1和Nrf-2在广泛的组织中表达。为了确定Nrf-1的功能，对Nrf-1基因进行了靶向破坏。纯合Nrf-1突变小鼠由于确定性红细胞生成中的非细胞自主性缺陷而贫血，并在子宫内死亡。
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