Chan J Y, Han X L, Kan Y W
Department of Laboratory Medicine, University of California, San Francisco 94143-0724.
Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11371-5. doi: 10.1073/pnas.90.23.11371.
We have devised a complementation assay in yeast to clone mammalian transcriptional activators and have used it to identify a human basic leucine-zipper transcription factor that we have designated Nrf1 for NF-E2-related factor 1. Nrf1 potentially encodes a 742-aa protein and displays marked homology to the mouse and human NF-E2 transcription factors. Nrf1 activates transcription via NF-E2 binding sites in yeast cells. The ubiquitous expression pattern of Nrf1 and the range of promoters containing the NF-E2 binding motif suggest that this gene may play a role in the regulation of heme synthesis and ferritin genes.
我们设计了一种酵母中的互补检测法来克隆哺乳动物转录激活因子，并利用该方法鉴定出一种人类碱性亮氨酸拉链转录因子，我们将其命名为Nrf1（NF-E2相关因子1）。Nrf1可能编码一种742个氨基酸的蛋白质，与小鼠和人类的NF-E2转录因子具有显著的同源性。Nrf1通过酵母细胞中的NF-E2结合位点激活转录。Nrf1普遍的表达模式以及含有NF-E2结合基序的启动子范围表明，该基因可能在血红素合成和铁蛋白基因的调控中发挥作用。
