Monda M, Viggiano A, Sullo A, De Luca V
Dipartimento di Fisiologia Umana e Funzioni Biologiche Integrate Filippo Bottazzi, Seconda Università degli Studi di Napoli, Italy.
Neuroscience. 1998 Apr;83(4):1239-43. doi: 10.1016/s0306-4522(97)00448-x.
The aim of the present experiment was to evaluate the role played by aspartic acid and glutamic acid of frontal cerebral cortex during the hyperthermia induced by prostaglandin E1. Two groups of six Sprague Dawley male rats were anaesthetized with ethyl-urethane. The frontal cortical concentrations of aspartic and glutamic acids, the firing rate of the sympathetic nerves to the interscapular brown adipose tissue, the colonic and interscapular brown adipose tissue temperatures were monitored both before and after an intracerebroventricular injection of prostaglandin E1 (500 ng) or saline. Aspartic and glutamic acids were collected using a microdialysis probe placed in the frontal cortex. Concentrations of aspartic and glutamic acids were measured by high-pressure liquid chromatography with fluorescence detector. Prostaglandin E1 induced an increase in the concentrations of aspartic and glutamic acids, in the firing rate of sympathetic nerves and in the colonic and interscapular brown adipose tissue temperatures. The findings of the present experiment indicate that an intracerebroventricular injection of prostaglandin E1 causes release of aspartic and glutamic acids in the frontal cortex.
本实验的目的是评估额叶皮质中的天冬氨酸和谷氨酸在前列腺素E1诱导的体温过高过程中所起的作用。两组共12只雄性斯普拉格-道利大鼠用氨基甲酸乙酯麻醉。在脑室内注射前列腺素E1（500纳克）或生理盐水前后，监测额叶皮质中天冬氨酸和谷氨酸的浓度、支配肩胛间棕色脂肪组织的交感神经的放电频率、结肠和肩胛间棕色脂肪组织的温度。使用置于额叶皮质的微透析探针收集天冬氨酸和谷氨酸。通过带荧光检测器的高压液相色谱法测量天冬氨酸和谷氨酸的浓度。前列腺素E1可导致天冬氨酸和谷氨酸浓度升高、交感神经放电频率增加以及结肠和肩胛间棕色脂肪组织温度升高。本实验结果表明，脑室内注射前列腺素E1会导致额叶皮质释放天冬氨酸和谷氨酸。
