Cruciani M, Malena M, Amalfitano G, Monti P, Bonomi L
Department of Infectious Diseases, University of Verona, Italy.
Clin Infect Dis. 1998 Feb;26(2):330-3. doi: 10.1086/516292.
Between September and October 1994 we observed three cases of Pseudomonas aeruginosa endophthalmitis in a single ophthalmology center. Endophthalmitis progressed rapidly following surgical intervention, and the three patients completely lost vision in the affected eye. Microbiological surveillance culture specimens were obtained from environmental sites, the operating team, intraocular lenses, irrigation fluids, and surgical equipment. P. aeruginosa was isolated from the internal tubing system of automated cataract surgical equipment. The strains of P. aeruginosa cultured from vitreous and anterior chamber specimens of case patients and from the surgical equipment were analyzed with pulsed-field gel electrophoresis. Genomic DNA typing of these isolates showed an identical banding pattern on ethidium bromide-stained gels. We believe that this is the first reported outbreak of P. aeruginosa endophthalmitis traced to automated surgical equipment. Genomic DNA typing emerged as a practical and reliable option for the epidemiological investigation of the outbreak.
1994年9月至10月期间，我们在一家眼科中心观察到3例铜绿假单胞菌性眼内炎病例。手术干预后，眼内炎迅速进展，3例患者患眼完全失明。从环境场所、手术团队、人工晶状体、冲洗液和手术设备中获取微生物监测培养标本。在自动白内障手术设备的内部管道系统中分离出铜绿假单胞菌。对从病例患者的玻璃体和前房标本以及手术设备中培养出的铜绿假单胞菌菌株进行脉冲场凝胶电泳分析。这些分离株的基因组DNA分型在溴化乙锭染色的凝胶上显示出相同的条带模式。我们认为这是首次报道的追溯至自动手术设备的铜绿假单胞菌性眼内炎暴发。基因组DNA分型成为此次暴发流行病学调查的实用且可靠的选择。
