Tang Y W, Hopkins M K, Kolbert C P, Hartley P A, Severance P J, Persing D H
Department of Laboratory Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA.
Clin Infect Dis. 1998 Feb;26(2):389-92. doi: 10.1086/516323.
We recovered an unusual bacterial strain from blood or sputum of three patients with septicemia, endocarditis, and/or respiratory failure. The three isolates were thin, curved, gram-negative, light brown, pigment-producing bacilli with variable catalase activity. They were asaccharolytic, oxidase-negative, nonmotile, and fastidious. Identification was not possible on the basis of these characteristics alone or in combination with cellular fatty acid profiles. Nucleic acid amplification and sequence analysis of the 16S rRNA gene revealed that all three isolates were identical and most closely related to the emerging pathogen Bordetella holmesii, diverging from the published sequence at three nucleotide positions (99.8% similarity). Isolation of a B. holmesii-like pathogen from sputum suggests that, in addition to producing septicemia, the organism may inhabit the respiratory tract like other Bordetella species.
我们从三名患有败血症、心内膜炎和/或呼吸衰竭的患者的血液或痰液中分离出一种不寻常的细菌菌株。这三株分离菌均为细长、弯曲、革兰氏阴性、浅棕色、产色素的杆菌，过氧化氢酶活性可变。它们不分解糖类、氧化酶阴性、无运动性且苛求营养。仅根据这些特征或结合细胞脂肪酸谱无法进行鉴定。对16S rRNA基因进行核酸扩增和序列分析表明，所有三株分离菌均相同，且与新出现的病原体霍氏博德特菌关系最为密切，在三个核苷酸位置与已发表序列存在差异（相似度为99.8%）。从痰液中分离出类似霍氏博德特菌的病原体表明，该生物体除了可引起败血症外，可能还像其他博德特菌属物种一样栖息于呼吸道。
