Weyant R S, Hollis D G, Weaver R E, Amin M F, Steigerwalt A G, O'Connor S P, Whitney A M, Daneshvar M I, Moss C W, Brenner D J
Emerging Bacterial and Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
J Clin Microbiol. 1995 Jan;33(1):1-7. doi: 10.1128/jcm.33.1.1-7.1995.
CDC nonoxidizer group 2 (NO-2) currently consists of 15 gram-negative, rod-shaped, oxidase-negative, asaccharolytic, brown soluble pigment-producing strains isolated from blood cultures, usually from young adults. On the basis of their cellular fatty acid profiles, NO-2 strains formed a single group that was identical with the profile of Bordetella avium. 16S rRNA sequencing of one NO-2 strain and the type strains of B. pertussis, B. parapertussis, B. bronchiseptica, and B. avium showed a high degree of homology (> or = 98% over 1,525 bases). The NO-2 guanine-plus-cytosine content (61.5 to 62.3 mol%) and major ubiquinone analysis (ubiquinone-8) results were both consistent with those for the genus Bordetella. DNA relatedness studies (hydroxyapatite method) confirmed a close relatedness between NO-2 and Bordetella species and demonstrated that NO-2 strains were a single new species. The name B. holmesii sp. nov. is proposed for CDC group NO-2.
美国疾病控制与预防中心非氧化酶2组（NO-2）目前由15株革兰氏阴性、杆状、氧化酶阴性、不分解糖类且能产生棕色可溶性色素的菌株组成，这些菌株通常从血液培养物中分离得到，且分离对象多为年轻人。基于其细胞脂肪酸谱，NO-2菌株形成了一个与鸟博德特氏菌谱相同的单一组群。对一株NO-2菌株以及百日咳博德特氏菌、副百日咳博德特氏菌、支气管败血博德特氏菌和鸟博德特氏菌的模式菌株进行16S rRNA测序，结果显示高度同源（在1525个碱基上同源性≥98%）。NO-2菌株的鸟嘌呤加胞嘧啶含量（61.5至62.3摩尔%）以及主要泛醌分析（泛醌-8）结果均与博德特氏菌属的结果一致。DNA相关性研究（羟基磷灰石法）证实了NO-2与博德特氏菌属各菌种之间存在密切相关性，并表明NO-2菌株是一个单一的新菌种。提议将美国疾病控制与预防中心NO-2组命名为霍氏博德特氏菌（B. holmesii）新种。
