Norlander T, Shimizu T, Larsen P, Westrin K M, Hultenby K, Carlsöö B, Stierna P
Department of Oto-Rhino-Laryngology, Karolinska Institute, Huddinge Hospital, Sweden.
Acta Otolaryngol. 1998 Jan;118(1):114-23. doi: 10.1080/00016489850155242.
To evaluate the pattern of goblet cell differentiation in sinus mucosa in response to external stimuli, New Zealand White rabbits were subjected to either experimental sinusitis or topical capsalcin application. Sinus mucosa was examined by light microscopy after serial sectioning, whole-mount preparation or immunohistochemistry. The mucosa was also examined by electron microscopy after perfusion fixation or high-pressure freezing. While goblet cells were normally very scarce in the healthy rabbit maxillary sinus mucosa, such cells were frequent after experimental sinusitis or topical capsaicin application. The process of goblet cell differentiation seems to follow a sequential path where serous secretory cells start to produce an increasing amount of mucous granules which appear electron lucent after conventional fixation. Parallel to this shift in secretion production, the cell assumes a bulging appearance after conventional fixation. It is concluded that newly formed goblet cells are recruited from intermediate secretory cell stages rather than from ciliated cells.
为评估鼻窦黏膜中杯状细胞对外界刺激的分化模式，将新西兰白兔分为实验组，分别诱发鼻窦炎或局部应用辣椒素。鼻窦黏膜通过连续切片、整装制片或免疫组织化学进行光学显微镜检查。在灌注固定或高压冷冻后，黏膜也通过电子显微镜检查。健康兔上颌窦黏膜中杯状细胞通常非常稀少，但在诱发鼻窦炎或局部应用辣椒素后，此类细胞变得频繁。杯状细胞分化过程似乎遵循一个连续的路径，浆液分泌细胞开始产生越来越多的黏液颗粒，在传统固定后这些颗粒呈现电子透明。与分泌产物的这种转变同时，在传统固定后细胞呈现凸起外观。得出的结论是，新形成的杯状细胞是从中分泌细胞阶段招募而来，而非来自纤毛细胞。
