人类肠道M细胞表现出类似肠上皮细胞的中间丝。

Human intestinal M cells exhibit enterocyte-like intermediate filaments.

作者信息

Kucharzik T, Lügering N, Schmid K W, Schmidt M A, Stoll R, Domschke W

机构信息

Department of Medicine B, University of Münster, Germany.

出版信息

Gut. 1998 Jan;42(1):54-62. doi: 10.1136/gut.42.1.54.

DOI:10.1136/gut.42.1.54

PMID:9505886

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1726964/

Abstract

BACKGROUND

The derivation and ultrastructural composition of M cells covering the lymphoid follicles of Peyer's patches is still unknown. Results from different animal models have shown that there are species specific differences in the composition of intermediate filaments between M cells and neighbouring enterocytes. Little is known, however, about intermediate filaments of human M cells.

AIMS

To compare components of the cytoskeleton of human M cells with those of adjacent absorptive enterocytes.

METHODS

The expression and localisation of different cytokeratins, vimentin, and desmin in M cells was determined on follicle associated epithelia of human appendix using immunohistochemistry and immunogold electron microscopy.

RESULTS

Cytokeratins specific for human intestinal epithelial cells such as cytokeratins 8, 18, 19, and 20 were expressed in both absorptive enterocytes and M cells with no differences in intensity and cellular distribution between both cell types. Vimentin and desmin, tissue specific markers of either mesenchymal or myogenic cells, as well as other cytokeratins were not detectable in enterocytes or M cells.

CONCLUSION

This is the first study on the structure of intermediate filaments in human intestinal M cells. Our results show that in contrast to several animal models, human M cells apparently do not differ from adjacent enterocytes in the composition of their intermediate filament cytoskeleton. The presence of enterocyte like cytokeratins and the absence of other cytokeratins as well as of vimentin and desmin supports the hypothesis of an epithelial origin of human intestinal M cells and suggests that M cells may derive from differentiated enterocytes.

摘要

背景

覆盖派尔集合淋巴结淋巴滤泡的微皱褶细胞（M细胞）的起源及其超微结构组成仍不清楚。不同动物模型的研究结果表明，M细胞与相邻肠上皮细胞之间中间丝的组成存在物种特异性差异。然而，关于人类M细胞的中间丝却知之甚少。

目的

比较人类M细胞与相邻吸收性肠上皮细胞的细胞骨架成分。

方法

采用免疫组织化学和免疫金电子显微镜技术，检测人阑尾滤泡相关上皮中不同细胞角蛋白、波形蛋白和平滑肌肌动蛋白在M细胞中的表达和定位。

结果

人类肠上皮细胞特异性的细胞角蛋白，如细胞角蛋白8、18、19和20，在吸收性肠上皮细胞和M细胞中均有表达，两种细胞类型在强度和细胞分布上无差异。波形蛋白和平滑肌肌动蛋白，间充质或肌源性细胞的组织特异性标志物，以及其他细胞角蛋白在肠上皮细胞或M细胞中均未检测到。

结论

这是第一项关于人类肠道M细胞中间丝结构的研究。我们的结果表明，与几种动物模型不同，人类M细胞在中间丝细胞骨架组成上与相邻肠上皮细胞明显没有差异。肠上皮样细胞角蛋白的存在以及其他细胞角蛋白、波形蛋白和平滑肌肌动蛋白的缺失支持了人类肠道M细胞起源于上皮细胞的假说，并表明M细胞可能来源于分化的肠上皮细胞。