Jepson M A, Mason C M, Bennett M K, Simmons N L, Hirst B H
Department of Physiological Sciences, University of Newcastle-upon-Tyne, Medical School, UK.
Histochem J. 1992 Jan;24(1):33-9. doi: 10.1007/BF01043285.
Membranous epithelial (M) cells within the follicle-associated epithelium which overlies gut-associated lymphoid tissue in Peyer's patches and of appendix have been shown by immunocytochemical staining, in rabbit, to contain both vimentin- and cytokeratin-type intermediate filaments. The specificity of vimentin immunostaining has been confirmed by blocking with purified vimentin and by immunoblotting. No evidence was obtained for the expression of vimentin in rat, mouse or human M cells. The possible significance of vimentin-expression in these specialized epithelial cells and the potential use of vimentin as a positive marker for M cells are discussed.
在派尔集合淋巴结和阑尾中覆盖肠道相关淋巴组织的滤泡相关上皮内的膜性上皮（M）细胞，经免疫细胞化学染色显示，在兔体内同时含有波形蛋白和细胞角蛋白类型的中间丝。波形蛋白免疫染色的特异性已通过用纯化的波形蛋白封闭以及免疫印迹得到证实。在大鼠、小鼠或人类的M细胞中未获得波形蛋白表达的证据。本文讨论了波形蛋白在这些特殊上皮细胞中表达的可能意义以及波形蛋白作为M细胞阳性标志物的潜在用途。
