子宫内膜异位症女性腹腔液中白细胞介素-12的水平。
The peritoneal fluid levels of interleukin-12 in women with endometriosis.
作者信息
Zeyneloglu H B, Senturk L M, Seli E, Bahtiyar O M, Olive D L, Arici A
机构信息
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT 06520-8063, USA.
出版信息
Am J Reprod Immunol. 1998 Feb;39(2):152-6. doi: 10.1111/j.1600-0897.1998.tb00347.x.
PROBLEM
Interleukin-12 (IL-12) is produced mainly by monocytes/macrophages, and it induces proliferation and cytotoxicity of T-cells and natural killer cells. In women with endometriosis, natural killer cell activity in the peritoneal fluid is significantly decreased. We aimed to measure the peritoneal fluid level of IL-12 in endometriosis.
METHOD OF STUDY
We measured IL-12 levels in peritoneal fluid samples from women with or without endometriosis and in supernatants from endometrial stromal, ovarian stromal, and mesothelial cell cultures, using a high-sensitivity enzyme-linked immunosorbent assay.
RESULTS
The median concentration of IL-12 in the peritoneal fluid of women with endometriosis was 1.1 pg/ml (range, 0.2-5.5) and was 1.6 pg/ml (range, 0.4-2.8) in women without endometriosis, not a statistically significant difference. IL-12 was not detected in the supernatants of endometrial stromal, ovarian stromal, and mesothelial cell cultures.
CONCLUSION
Concentrations of IL-12 in the peritoneal fluid of women with or without endometriosis are low, but they are detectable and are not affected significantly by the presence of endometriosis.
问题
白细胞介素 -12（IL -12）主要由单核细胞/巨噬细胞产生，可诱导T细胞和自然杀伤细胞的增殖及细胞毒性。在子宫内膜异位症女性患者中，腹腔液中的自然杀伤细胞活性显著降低。我们旨在测定子宫内膜异位症患者腹腔液中IL -12的水平。
研究方法
我们使用高灵敏度酶联免疫吸附测定法，测量了患有或未患有子宫内膜异位症女性的腹腔液样本以及子宫内膜基质细胞、卵巢基质细胞和间皮细胞培养上清液中的IL -12水平。
结果
患有子宫内膜异位症女性腹腔液中IL -12的中位浓度为1.1 pg/ml（范围为0.2 - 5.5），未患子宫内膜异位症症女性的为1.6 pg/ml（范围为0.4 - 2.8），差异无统计学意义。在子宫内膜基质细胞、卵巢基质细胞和间皮细胞培养上清液中未检测到IL -12。
结论
患有或未患有子宫内膜异位症女性的腹腔液中IL -12浓度较低，但可检测到，且不受子宫内膜异位症存在的显著影响。
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