检测和鉴定源自OXA-10的头孢他啶水解超广谱β-内酰胺酶的实用方法
Practical approach for detection and identification of OXA-10-derived ceftazidime-hydrolyzing extended-spectrum beta-lactamases.
作者信息
Vahaboglu H, Ozturk R, Akbal H, Saribas S, Tansel O, Coşkunkan F
机构信息
Kocaeli Universitesi, Tip Fakültesi, Turkey.
出版信息
J Clin Microbiol. 1998 Mar;36(3):827-9. doi: 10.1128/JCM.36.3.827-829.1998.
Abstract
A practical approach to detect and identify ceftazidime-hydrolyzing extended-spectrum mutants of OXA-10 beta-lactamase is presented. Large numbers of bacteria were screened by colony hybridization, a 720-bp part of blaOXA was amplified by PCR from the hybridization-positive isolates, and the products were digested by PvuII and HaeIII.
摘要
本文介绍了一种检测和鉴定水解头孢他啶的OXA-10超广谱β-内酰胺酶突变体的实用方法。通过菌落杂交筛选大量细菌，从杂交阳性分离株中通过PCR扩增blaOXA的720bp片段，并将产物用PvuII和HaeIII进行酶切。
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