Vahaboglu H, Oztürk R, Aygün G, Coşkunkan F, Yaman A, Kaygusuz A, Leblebicioglu H, Balik I, Aydin K, Otkun M
Infectious Diseases and Clinical Microbiology Department, Kocaeli University Medical School, Turkey.
Antimicrob Agents Chemother. 1997 Oct;41(10):2265-9. doi: 10.1128/AAC.41.10.2265.
We studied the prevalence and molecular epidemiology of PER-1-type beta-lactamases among Acinetobacter, Klebsiella, and Pseudomonas aeruginosa strains isolated over a 3-month period in eight university hospitals from distinct regions of Turkey. A total of 72, 92, and 367 Acinetobacter, Klebsiella, and P. aeruginosa isolates were studied, respectively. The presence of blaPER was determined by the colony hybridization method and later confirmed by isoelectric focusing. We detected PER-1-type beta-lactamases in 46% (33/72) of Acinetobacter strains and in 11% (40/367) of P. aeruginosa strains but not in Klebsiella strains. PER-1-type enzyme producers were highly resistant to ceftazidime and gentamicin, intermediately resistant to amikacin, and susceptible or moderately susceptible to imipenem and meropenem. Among PER-1-type-beta-lactamase-positive isolates, five Acinetobacter isolates and six P. aeruginosa isolates from different hospitals were selected for ribosomal DNA fingerprinting with EcoRI and SalI. The EcoRI-digested DNAs were later hybridized with a digoxigenin-labelled PER-1 probe. The ribotypes and the lengths of blaPER-carrying fragments were identical in four Acinetobacter strains. A single isolate (Ac3) harbored a PER gene on a different fragment (approximately 4.2 kbp) than the others (approximately 3.4 kbp) and showed a clearly distinguishable ribotype. Ribotypes of P. aeruginosa strains obtained with EcoRI showed three patterns. Similarly, in Pseudomonas strains two different EcoRI fragments harbored blaPER (approximately 4.2 kbp in five isolates and 3.4 kbp in one isolate). PER-1-type beta-lactamases appear to be restricted to Turkey. However, their clonal diversity and high prevalence indicate a high spreading potential.
我们研究了在土耳其不同地区的八所大学医院中，为期3个月分离出的不动杆菌、克雷伯菌和铜绿假单胞菌菌株中PER-1型β-内酰胺酶的流行情况和分子流行病学。分别对72株不动杆菌、92株克雷伯菌和367株铜绿假单胞菌分离株进行了研究。通过菌落杂交法确定blaPER的存在，随后通过等电聚焦进行确认。我们在46%（33/72）的不动杆菌菌株和11%（40/367）的铜绿假单胞菌菌株中检测到了PER-1型β-内酰胺酶，但在克雷伯菌菌株中未检测到。PER-1型酶产生菌对头孢他啶和庆大霉素高度耐药，对阿米卡星中度耐药，对亚胺培南和美罗培南敏感或中度敏感。在PER-1型β-内酰胺酶阳性分离株中，从不同医院选取了5株不动杆菌分离株和6株铜绿假单胞菌分离株，用EcoRI和SalI进行核糖体DNA指纹图谱分析。随后，用洋地黄毒苷标记的PER-1探针与EcoRI消化的DNA进行杂交。在4株不动杆菌菌株中，核糖体类型和携带blaPER片段的长度相同。单个分离株（Ac3）携带PER基因的片段（约4.2 kbp）与其他分离株（约3.4 kbp）不同，显示出明显可区分的核糖体类型。用EcoRI获得的铜绿假单胞菌菌株的核糖体类型显示出三种模式。同样，在假单胞菌菌株中，两个不同的EcoRI片段携带blaPER（5株分离株中约为4.2 kbp，1株分离株中约为3.4 kbp）。PER-1型β-内酰胺酶似乎仅限于土耳其。然而，它们的克隆多样性和高流行率表明其具有很高的传播潜力。
