Kobayashi K, Rabinowicz P, Bravo-Almonacid F, Helguera M, Conci V, Lot H, Mentaberry A
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, CONICET, Buenos Aires, Argentina.
Arch Virol. 1996;141(12):2277-87. doi: 10.1007/BF01718630.
Partial genomic sequences from an unknown garlic potyvirus and from an onion isolate of the onion yellow dwarf potyvirus (OYDV) were obtained. Comparison of the deduced amino acid sequences showed a similarity of 88% between the respective viral coat proteins. The garlic potyvirus coat protein was expressed in E. coli cells, purified, and subjected to Western blot analysis using antibodies raised against different garlic-infecting viruses. The expression protein was consistently recognised by anti-OYDV antibodies and did not react with antibodies specific for leek yellow stripe potyvirus (LYSV), garlic common latent carlavirus (GCLV) and shallot latent carlavirus (SLV). Besides, the garlic potyvirus coat protein was obtained as a fusion protein and used as antigen to produce polyclonal antibodies. These antibodies reacted with purified OYDV virions, but failed to recognise LYSV particles. In the light of this evidence the garlic potyvirus was identified as the garlic strain of OYDV.
获得了一种未知大蒜马铃薯Y病毒和洋葱黄矮马铃薯Y病毒（OYDV）洋葱分离株的部分基因组序列。推导的氨基酸序列比较显示，相应病毒外壳蛋白之间的相似度为88%。大蒜马铃薯Y病毒外壳蛋白在大肠杆菌细胞中表达、纯化，并使用针对不同感染大蒜病毒产生的抗体进行蛋白质印迹分析。表达蛋白始终被抗OYDV抗体识别，且不与韭菜黄条病毒（LYSV）、大蒜普通潜隐香石竹潜隐病毒（GCLV）和青葱潜隐香石竹潜隐病毒（SLV）的特异性抗体发生反应。此外，大蒜马铃薯Y病毒外壳蛋白作为融合蛋白获得，并用作抗原产生多克隆抗体。这些抗体与纯化的OYDV病毒粒子发生反应，但未能识别LYSV粒子。根据这些证据，大蒜马铃薯Y病毒被鉴定为OYDV的大蒜株系。
