Shaarawy M, Aref A, Salem M E, Sheiba M
Department of Obstetrics and Gynecology, Cairo University, Egypt.
Int J Gynaecol Obstet. 1998 Feb;60(2):123-8. doi: 10.1016/s0020-7292(97)00256-7.
Increased free-radical activity may be implicated in the pathogenesis of pre-eclampsia and knowledge of the response of the antioxidant systems is limited in pre-eclampsia and lacking in eclampsia. Accordingly, this study was initiated to assess total antioxidant status in pre-eclampsia and eclampsia.
Thirty-five patients with pre-eclampsia (20 mild and 15 severe), 10 patients with eclampsia and 20 normotensive women (controls) with singleton gestations in the third trimester participated in this study. Their freshly drawn blood was assayed for total antioxidant status colorimetrically using incubation mixture of ABTS, a peroxidase (metmyoglobin) and H2O2. In addition, serum fibronectin was determined by radial immunodiffusion.
Serum total antioxidant status levels in mild and severe pre-eclampsia and eclampsia were significantly lower than that of healthy pregnant women (controls). The mean percent decreases amounted to 22%, 40% and 59%, respectively, indicating the propensity of these patients to oxidative stress.
These results demonstrate that radical-scavenging antioxidants are consumed by the increased free-radical activity in pre-eclampsia. The mechanisms by which free radicals are involved in endothelial dysfunction and renal injury are explained as evidenced by significant negative correlation of fibronectin and positive correlation of creatinine clearance with total antioxidants.
自由基活性增加可能与子痫前期的发病机制有关，而子痫前期抗氧化系统的反应相关知识有限，子痫患者则完全缺乏这方面的了解。因此，本研究旨在评估子痫前期和子痫患者的总抗氧化状态。
35例子痫前期患者（20例轻度和15例重度）、10例子痫患者以及20例孕晚期单胎妊娠的血压正常女性（对照组）参与了本研究。采用ABTS、过氧化物酶（高铁肌红蛋白）和过氧化氢的孵育混合物，通过比色法测定他们新鲜采集血液中的总抗氧化状态。此外，采用放射免疫扩散法测定血清纤连蛋白。
轻度和重度子痫前期以及子痫患者的血清总抗氧化状态水平显著低于健康孕妇（对照组）。平均下降百分比分别为22%、40%和59%，表明这些患者易发生氧化应激。
这些结果表明，子痫前期自由基活性增加消耗了清除自由基的抗氧化剂。纤连蛋白与总抗氧化剂呈显著负相关，肌酐清除率与总抗氧化剂呈正相关，这解释了自由基参与内皮功能障碍和肾损伤的机制。
