Bienvenu J, Graziani M S, Arpin F, Bernon H, Blessum C, Marchetti C, Righetti G, Somenzini M, Verga G, Aguzzi F
Laboratoire d'Immunologie, Centre Hospitalier Lyon Sud, Pierre-Bénite, France.
Clin Chem. 1998 Mar;44(3):599-605.
Serum protein electrophoresis and typing of monoclonal components (MCs) are routine but time-consuming and technically demanding assays. We evaluated capillary electrophoresis (Paragon CZE 2000) for automation of the two assays. CZE and cellulose acetate electrophoresis gave similar data on 794 samples. Within-run and between-run CVs were < 2% for albumin and gamma-globulins and 4-7% for alpha 1-, alpha 2-, and beta-globulins. Bilirubin, hemoglobin, triglycerides, and fibrinogen were found not to interfere. No carryover by capillaries was detected. The detection limit for MC was < 0.5 g/L. MC assessment by immunosubtraction on 403 samples identified the monoclonal type in all samples with peak concentrations > 10 g/L; only 50% of MCs that could not be quantified by densitometric scan were typed.
血清蛋白电泳及单克隆成分（MCs）分型是常规检测，但耗时且技术要求高。我们评估了毛细管电泳（Paragon CZE 2000）用于这两项检测的自动化。毛细管区带电泳（CZE）和醋酸纤维素电泳对794份样本得出了相似的数据。白蛋白和γ球蛋白的批内及批间变异系数（CV）<2%，α1、α2和β球蛋白的CV为4 - 7%。发现胆红素、血红蛋白、甘油三酯和纤维蛋白原无干扰。未检测到毛细管的残留。MC的检测限<0.5 g/L。对403份样本进行免疫扣除法MC评估，在所有峰值浓度>10 g/L的样本中均鉴定出单克隆类型；通过密度扫描无法定量的MC中只有50%进行了分型。
