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[Restriction endonuclease Sse9I (correction of Sse91) from Sporosarcina strain SP.9D recognizes the 5'-AATT-3' DNA sequence].

作者信息

Gonchar D A, Dedkov V S, Verkhozina V A, Kusner Iu S, Shevchenko A V, Degtiarev S Kh

出版信息

Mol Gen Mikrobiol Virusol. 1998(1):32-4.

PMID:9511140
Abstract

Sse91, a type II restriction endonuclease, has been isolated from Sporosarcina species 9D. The recognition sequence and cleavage point of restriction endonuclease Sse91 have been determined as 5'-decrease AATT-3'. The new enzyme is an isoschizomer of Tsp5091, but its optimal incubation temperature is 55 degrees C and it is inactivated at 65 degrees C for 20 min.

摘要

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