识别5'-AATT-3'的Sse9I DNA甲基转移酶的克隆与特性分析

Cloning and characterization of Sse9I DNA-methyltransferase recognizing 5'-AATT-3'.

作者信息

Gonchar D A, Wolf Y I, Degtyarev S Kh

机构信息

SibEnzyme, Novosibirsk, Russia.

出版信息

Nucleic Acids Res. 1996 Jul 15;24(14):2790-2. doi: 10.1093/nar/24.14.2790.

DOI:10.1093/nar/24.14.2790

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC146017/

Abstract

The gene from Sporosarcina species 9D encoding Sse9I DNA-methyltransferase (M.Sse9I) was cloned and expressed in Escherichia coli. The recombinant plasmid pMSse-1 contains the M.Sse9I gene 1086 bp in length, corresponding to a protein of 362 amino acid residues. M.Sse9I recognizes the tetranucleotide sequence 5'-AATT-3' and modifies the second adenine within the recognition sequence. The amino acid sequence of M.Sse9I was compared with those of other methylases. According to mutual positions of four conservative domains the new enzyme belongs to a subgroup of D12 class. This subgroup includes Sse9I, CviAII, NlaIII and N-terminal domains of LlaI, FokI and StsI DNA-methyltransferases.

摘要

克隆了来自嗜盐芽孢杆菌属9D种编码Sse9I DNA甲基转移酶（M.Sse9I）的基因，并在大肠杆菌中进行表达。重组质粒pMSse-1含有长度为1086 bp的M.Sse9I基因，对应于一个由362个氨基酸残基组成的蛋白质。M.Sse9I识别四核苷酸序列5'-AATT-3'，并修饰识别序列中的第二个腺嘌呤。将M.Sse9I的氨基酸序列与其他甲基化酶的氨基酸序列进行了比较。根据四个保守结构域的相互位置，该新酶属于D12类的一个亚组。该亚组包括Sse9I、CviAII、NlaIII以及LlaI、FokI和StsI DNA甲基转移酶的N端结构域。

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