Gómez-Lus M L, Giménez M J, Prieto J, Martín M, Frías J, Aguilar L
Microbiology Department, School of Medicine, Universidad Complutense, Madrid, Spain.
Eur J Clin Microbiol Infect Dis. 1998 Jan;17(1):40-3. doi: 10.1007/BF01584362.
The effect of phagocytic killing on serum bactericidal activity against Streptococcus pneumoniae was investigated 0, 1.5, 8 and 12 h after a single 875 mg oral dose of amoxicillin in healthy adults. Killing curves were determined with polymorphonuclear neutrophils (PMN), serum or PMN plus serum. Global killing (i. e. intracellular and extracellular killing) over 3 h of incubation was expressed as the area under the killing curve (AUKC; log cfu x h/ml). Amoxicillin did not affect the activity of PMN alone. For serum alone, the AUKC of post-administration samples (with supra-inhibitory amoxicillin concentrations) was significantly lower than in baseline samples. For serum plus PMN, significant bactericidal activity of serum was still found in samples after antibiotic concentrations had reached sub-inhibitory levels.
在健康成年人单次口服875毫克阿莫西林后0、1.5、8和12小时，研究了吞噬杀伤对血清针对肺炎链球菌的杀菌活性的影响。用多形核中性粒细胞（PMN）、血清或PMN加血清测定杀伤曲线。3小时孵育期间的总体杀伤（即细胞内和细胞外杀伤）表示为杀伤曲线下面积（AUKC；log cfu×h/ml）。阿莫西林单独不影响PMN的活性。对于单独血清，给药后样本（阿莫西林浓度高于抑制浓度）的AUKC显著低于基线样本。对于血清加PMN，在抗生素浓度达到亚抑制水平后的样本中仍发现血清具有显著杀菌活性。
