Rufas O, Gilman A, Fisch B, Shalgi R
Department of Embryology and Teratology, Sackler School of Medicine, Tel-Aviv University, Ramat Aviv, Israel.
J Assist Reprod Genet. 1998 Feb;15(2):84-9. doi: 10.1007/BF02766831.
One of the most challenging and intriguing groups of infertile patients is that of normozoospermic men who have repeatedly not achieved fertilization in vitro. These cases probably present a wide range of gamete disorders manifested at different stages of the fertilization process. The occurrence of spontaneous and follicular fluid (FF)-induced acrosome reactions (ARs) in in vitro fertilizing and nonfertilizing specimens from normozoospermic IVF patients was assessed, and the effect of Percoll and cryopreservation on the incidence of ARs was evaluated.
Semen samples from 62 normozoospermic (15 in vitro nonfertilizing and 47 fertilizing) patients were analyzed. Spermatozoa were double-stained with FITC-conjugated Pissum sativum lectin, to assess their acrosomal status, and propidium iodide, to evaluate their vitality, using flow cytometry analysis.
A lower average of AR incidence was observed in the nonfertilizing than in the fertilizing group with all treatments. Both groups exhibited an increase in the proportion of ARs following incubation with FF. This rise was most prominent when Percoll-separated fractions were used (15.8 and 25.6% AR in the nonfertilizing and fertilizing groups, respectively). Thawed cryopreserved and fresh fertilizing samples exhibited similar AR rates.
That normozoospermic recurrently nonfertilizing compared to fertilizing semen samples have a lower capacity to undergo ARs is suggested.
在不育患者中，最具挑战性且引人关注的群体之一是精子正常但多次体外受精未成功的男性。这些病例可能存在多种在受精过程不同阶段表现出来的配子异常。评估了正常精子形态的体外受精患者的体外受精和未受精样本中自发顶体反应（AR）以及卵泡液（FF）诱导的顶体反应的发生率，并评估了Percoll和冷冻保存对顶体反应发生率的影响。
分析了62例精子正常的患者（15例体外受精未成功和47例体外受精成功）的精液样本。使用流式细胞术分析，用异硫氰酸荧光素（FITC）偶联的豌豆凝集素对精子进行双重染色，以评估其顶体状态，并用碘化丙啶评估其活力。
在所有处理中，未受精组的顶体反应发生率平均低于受精组。两组在与卵泡液孵育后顶体反应的比例均有所增加。当使用Percoll分离的组分时，这种增加最为显著（未受精组和受精组的顶体反应分别为15.8%和25.6%）。解冻的冷冻保存样本和新鲜的受精样本表现出相似的顶体反应率。
提示与受精精液样本相比，精子正常但反复未受精的精液样本发生顶体反应的能力较低。
