Ogg G S, Jin X, Bonhoeffer S, Dunbar P R, Nowak M A, Monard S, Segal J P, Cao Y, Rowland-Jones S L, Cerundolo V, Hurley A, Markowitz M, Ho D D, Nixon D F, McMichael A J
Institute of Molecular Medicine, Nuffield Department of Medicine, Oxford OX3 9DS, UK.
Science. 1998 Mar 27;279(5359):2103-6. doi: 10.1126/science.279.5359.2103.
Although cytotoxic T lymphocytes (CTLs) are thought to be involved in the control of human immunodeficiency virus-type 1 (HIV-1) infection, it has not been possible to demonstrate a direct relation between CTL activity and plasma RNA viral load. Human leukocyte antigen-peptide tetrameric complexes offer a specific means to directly quantitate circulating CTLs ex vivo. With the use of the tetrameric complexes, a significant inverse correlation was observed between HIV-specific CTL frequency and plasma RNA viral load. In contrast, no significant association was detected between the clearance rate of productively infected cells and frequency of HIV-specific CTLs. These data are consistent with a significant role for HIV-specific CTLs in the control of HIV infection and suggest a considerable cytopathic effect of the virus in vivo.
尽管细胞毒性T淋巴细胞（CTLs）被认为参与了对人类免疫缺陷病毒1型（HIV-1）感染的控制，但尚未能够证明CTL活性与血浆RNA病毒载量之间存在直接关系。人类白细胞抗原肽四聚体复合物提供了一种在体外直接定量循环CTLs的特异性方法。使用四聚体复合物时，观察到HIV特异性CTL频率与血浆RNA病毒载量之间存在显著的负相关。相比之下，在有生产性感染细胞的清除率与HIV特异性CTL频率之间未检测到显著关联。这些数据与HIV特异性CTLs在控制HIV感染中发挥重要作用一致，并提示该病毒在体内具有相当大的细胞病变效应。
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