Herulf M, Ljung T, Hellström P M, Weitzberg E, Lundberg J O
Dept. of Pharmacology, Karolinska Institute, Stockholm, Sweden.
Scand J Gastroenterol. 1998 Feb;33(2):164-9. doi: 10.1080/00365529850166897.
The production of nitric oxide (NO) is increased in ulcerative colitis, as shown by bioassays of NO synthase activity in mucosal biopsy specimens. We wanted to develop a less invasive method for measurement of NO directly in the rectum in patients with inflammatory bowel disease (IBD).
We studied 10 patients with active ulcerative colitis, 6 with active Crohn's disease, 6 with non-active ulcerative colitis, and 24 controls without inflammation A tonometer balloon catheter was inserted in the rectum and inflated with 5 ml of NO-free air. After 15 min of incubation the sample was extracted, and the NO concentration was immediately analysed with a chemiluminescence technique.
Patients with active disease had greatly increased concentrations of NO in the rectum as compared with controls and patients with non-active disease.
During inflammation in the large intestine increased amounts of NO are released from the mucosa. Measurements of NO directly in the rectum could be of help in further understanding the role of this gas in IBD. Moreover, it is tempting to speculate that this minimally invasive method could be clinically useful as a diagnostic tool and in monitoring the effect of therapy.
如黏膜活检标本中一氧化氮合酶活性的生物测定所示，溃疡性结肠炎中一氧化氮（NO）的生成增加。我们希望开发一种侵入性较小的方法，用于直接测量炎症性肠病（IBD）患者直肠中的NO。
我们研究了10例活动期溃疡性结肠炎患者、6例活动期克罗恩病患者、6例非活动期溃疡性结肠炎患者以及24例无炎症的对照者。将眼压计球囊导管插入直肠，并用5 ml无NO的空气充气。孵育15分钟后提取样本，并立即用化学发光技术分析NO浓度。
与对照者和非活动期疾病患者相比，活动期疾病患者直肠中的NO浓度大幅增加。
在大肠炎症期间，黏膜释放的NO量增加。直接测量直肠中的NO可能有助于进一步了解这种气体在IBD中的作用。此外，很容易推测这种微创方法作为一种诊断工具以及监测治疗效果在临床上可能有用。
