Hughes P S, Hughes A P
J Am Acad Dermatol. 1998 Mar;38(3):426-8. doi: 10.1016/s0190-9622(98)70500-6.
The erbium:YAG laser (Continuum Biomedical, Dublin, Calif.) is a new resurfacing and ablating laser that produces minimal residual thermal damage. Laser safety requires careful attention to the hazards of the laser plume. It is important to know whether viable organisms survive in the vapors. Human papillomavirus (HPV) DNA has been detected in the vapor of carbon dioxide laser-treated and electrodesiccated human warts. The presence or absence of HPV DNA in the laser plume of erbium:YAG laser-treated warts has not been previously studied to our knowledge.
Our purpose was to determine the presence or absence of HPV DNA in the laser plume of erbium:YAG laser-treated human warts.
One half of clinically typical and histopathologically confirmed verrucae vulgares from five patients were submitted for HPV DNA detection with in situ hybridization. After erbium:YAG laser ablation of the remainder of the warts, the laser plume was deposited on the handpiece as an abundant fluffy material and was submitted for evaluation of HPV DNA by polymerase chain reaction with consensus primers for the HPV type detected in the wart specimens.
HPV2 DNA was found in all warts. HPV DNA was not detected in the erbium:YAG laser plume after ablation of these same warts.
The absence of HPV DNA in the plume of erbium:YAG laser-treated warts is a significant safety feature of this laser.
铒钇铝石榴石激光（Continuum Biomedical公司，加利福尼亚州都柏林）是一种新型的用于皮肤磨削和消融的激光，产生的残余热损伤极小。激光安全需要特别注意激光羽流的危害。了解蒸汽中是否存在存活的生物体很重要。在二氧化碳激光治疗和电干燥的人类疣的蒸汽中已检测到人类乳头瘤病毒（HPV）DNA。据我们所知，此前尚未研究过铒钇铝石榴石激光治疗疣的激光羽流中HPV DNA的存在与否。
我们的目的是确定铒钇铝石榴石激光治疗人类疣的激光羽流中是否存在HPV DNA。
对5例临床典型且经组织病理学确诊的寻常疣患者，取一半疣体进行原位杂交检测HPV DNA。对其余疣体进行铒钇铝石榴石激光消融后，激光羽流以丰富的蓬松物质形式沉积在手持件上，并通过聚合酶链反应使用在疣体标本中检测到的HPV类型的通用引物对其进行HPV DNA评估。
所有疣体中均发现HPV2 DNA。在对这些相同的疣体进行消融后，铒钇铝石榴石激光羽流中未检测到HPV DNA。
铒钇铝石榴石激光治疗疣的羽流中不存在HPV DNA是该激光的一个重要安全特性。
