使用新型UL37外显子3和US3引物对临床标本中的人巨细胞病毒DNA进行PCR检测。
PCR detection of human cytomegalovirus DNA in clinical specimens using novel UL37 exon 3 and US3 primers.
作者信息
Lesperance M M, Contopoulos-Ioannidis D G, del Pilar Gutiérrez M, Colberg-Poley A M
机构信息
Center for Virology, Immunology, and Infectious Disease Research, Department of Otolaryngology, Children's National Medical Center, Washington, DC 20010, USA.
出版信息
Clin Diagn Lab Immunol. 1998 Mar;5(2):256-8. doi: 10.1128/CDLI.5.2.256-258.1998.
Abstract
The sensitivity and specificity of novel UL37 exon 3 (UL37x3) and US3 immediate-early (IE) gene PCR primers to detect human cytomegalovirus (HCMV) DNA in clinical specimens are comparable to those of HCMV DNA polymerase (UL54) primers. The use of these IE primers increases the diagnostic performance of HCMV PCR.
摘要
新型UL37外显子3（UL37x3）和US3立即早期（IE）基因PCR引物检测临床标本中人巨细胞病毒（HCMV）DNA的敏感性和特异性与HCMV DNA聚合酶（UL54）引物相当。使用这些IE引物可提高HCMV PCR的诊断性能。
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