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接触镜佩戴者的泪液抗坏血酸水平和总抗氧化状态：一项初步研究。

Tear ascorbic acid levels and the total antioxidant status in contact lens wearers: a pilot study.

机构信息

Biochemistry Research Department, Sankara Nethralaya, Vision and Medical Research Foundation, 18 College Road, Chennai, India.

出版信息

Indian J Ophthalmol. 2009 Jul-Aug;57(4):289-92. doi: 10.4103/0301-4738.53054.

DOI:10.4103/0301-4738.53054
PMID:19574697
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2712698/
Abstract

AIMS

The tear ascorbate owing to its high concentration, functions as an effective antioxidant against the oxidative damage of cornea. Contact lens wearers (CLW) are prone to oxidative stress due to the lens-induced hypoxic conditions. A pilot study was done to compare the tear ascorbic acid level and the total antioxidant capacity give as in normal and CLW.

MATERIALS AND METHODS

In this study 21 CLW (Mean age 23 +/- 3 years ; M-2, F-19), who were daily wear users, with duration of wear not more than four years, along with age-matched 28 controls (Mean age 28 +/- 3 ; M-15, F-13) were recruited in the study for collection of reflex tears using Schirmer's strip. Ascorbic acid in tears was determined using high-performance liquid chromatography (HPLC), total antioxidant capacity (TAC) and total protein assay by spectrophotometric analysis.

RESULTS

CLW showed no significant change in the tear ascorbic acid levels (0.4 +/- 0.26 mM) compared to the control subjects (0.61 +/- 0.59 mM). The amount of ascorbic acid in tears did not correlate with the TAC or the total protein of the tears. The mean TAC in CLW was 0.69 +/- 0.16 mM, with a total protein of 1.35 +/- 0.46 mg/ml while in controls it was 0.7 +/- 0.18 mM and 1.21 +/- 0.47 mg/ml respectively.

CONCLUSIONS

Soft contact lens wear did not show any significant change in tear ascorbic acid, TAC and total protein levels compared to controls.

摘要

目的

由于浓度高，眼泪中的抗坏血酸作为角膜氧化损伤的有效抗氧化剂。由于镜片诱导的缺氧条件，隐形眼镜佩戴者（CLW）容易受到氧化应激。进行了一项初步研究，以比较正常和 CLW 中的泪液抗坏血酸水平和总抗氧化能力。

材料和方法

在这项研究中，招募了 21 名 CLW（平均年龄 23 +/- 3 岁；M-2，F-19），他们是日常佩戴者，佩戴时间不超过四年，以及年龄匹配的 28 名对照者（平均年龄 28 +/- 3；M-15，F-13），用于使用 Schirmer 条收集反射泪液。使用高效液相色谱法（HPLC）测定泪液中的抗坏血酸，分光光度法测定总抗氧化能力（TAC）和总蛋白。

结果

CLW 的泪液抗坏血酸水平（0.4 +/- 0.26 mM）与对照受试者（0.61 +/- 0.59 mM）相比没有显著变化。泪液中抗坏血酸的量与 TAC 或泪液总蛋白无关。CLW 的平均 TAC 为 0.69 +/- 0.16 mM，总蛋白为 1.35 +/- 0.46 mg/ml，而对照组分别为 0.7 +/- 0.18 mM 和 1.21 +/- 0.47 mg/ml。

结论

与对照组相比，软性隐形眼镜佩戴者的泪液抗坏血酸、TAC 和总蛋白水平没有明显变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5f3/2712698/9d9894383b6b/IJO-57-289-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5f3/2712698/1395221dc917/IJO-57-289-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5f3/2712698/9d9894383b6b/IJO-57-289-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5f3/2712698/1395221dc917/IJO-57-289-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5f3/2712698/9d9894383b6b/IJO-57-289-g002.jpg

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