Khare V, Sodhi A, Singh S M
School of Biotechnology, Banaras Hindu University, Varanasi, India.
Nat Immun. 1996;15(6):285-94.
The present investigation was carried out to study the effect of aging on the activation of murine peritoneal macrophages by lipopolysaccharide (LPS) plus interferon-gamma (IFN-gamma) to tumoricidal state. Age-dependent alteration in macrophage-mediated tumor cell binding, cytotoxicity, cytostasis and production of the tumoricidal effector molecules oncostatin M, tumor necrosis factor and nitric oxide (NO) was studied. Macrophages (1.5 x 10(5)) obtained from mice of different age groups were separated on the basis of the reproductive status of the mice into prereproductive (young), reproductive (adult) and postreproductive (old) for studying their activation. Macrophages obtained from the old mice were found to be least responsive to the activation signal of LPS + IFN-gamma for macrophage-mediated tumoricidal activity and production of effector molecules. The macrophages of the old mice did not express inducible nitric oxide synthase, an enzyme responsible for the production of NO by activated macrophages. Macrophages of the adult and young groups showed better response; however, optimal response was observed in the macrophages of adult mice. The reasons for the observed difference in the response of macrophages are discussed.
本研究旨在探讨衰老对脂多糖（LPS）加干扰素-γ（IFN-γ）激活小鼠腹腔巨噬细胞至杀瘤状态的影响。研究了巨噬细胞介导的肿瘤细胞结合、细胞毒性、细胞生长抑制以及抑瘤素M、肿瘤坏死因子和一氧化氮（NO）等杀瘤效应分子产生方面的年龄依赖性变化。根据小鼠的生殖状态，将不同年龄组小鼠获取的巨噬细胞（1.5×10⁵）分为生殖前期（幼年）、生殖期（成年）和生殖后期（老年），以研究其激活情况。发现老年小鼠的巨噬细胞对LPS + IFN-γ的激活信号在巨噬细胞介导的杀瘤活性和效应分子产生方面反应最弱。老年小鼠的巨噬细胞不表达诱导型一氧化氮合酶，该酶负责激活的巨噬细胞产生NO。成年组和幼年组的巨噬细胞反应较好；然而，成年小鼠的巨噬细胞观察到最佳反应。讨论了观察到的巨噬细胞反应差异的原因。
