从聚球藻属蓝细菌PCC6301菌株及相关生物中鉴定10Sa RNA（转运信使RNA）同源物。

Identification of 10Sa RNA (tmRNA) homologues from the cyanobacterium Synechococcus sp. strain PCC6301 and related organisms.

作者信息

Watanabe T, Sugita M, Sugiura M

机构信息

Center for Gene Research, Nagoya University, Japan.

出版信息

Biochim Biophys Acta. 1998 Mar 4;1396(1):97-104. doi: 10.1016/s0167-4781(97)00180-2.

DOI:10.1016/s0167-4781(97)00180-2

PMID:9524235

Abstract

We have isolated the 10Sa RNA (tmRNA) from the unicellular cyanobacterium Synechococcus sp. strain PCC6301. It comprises of 394 nucleotides (nt) and has 55% homology to Escherichia coli tmRNA. The cloning and sequencing of the corresponding gene have revealed that, like in many tRNA genes, the terminal CCA sequence reported in all the tmRNA species characterized so far is not encoded in the DNA. Hybridization analysis has shown that the tmRNA gene is present as a single copy. Fairly high levels of tmRNA accumulate throughout the cell cycle; however, a slight increase in its level is observed during late-log to stationary phase. This suggests that tmRNA is functional not only when cells divide actively but also when cell growth stops.

摘要

我们已从单细胞蓝藻聚球藻属（Synechococcus sp.）菌株PCC6301中分离出10Sa RNA（转移信使RNA）。它由394个核苷酸（nt）组成，与大肠杆菌转移信使RNA具有55%的同源性。相应基因的克隆和测序表明，与许多tRNA基因一样，迄今为止所有已鉴定的转移信使RNA物种中报道的末端CCA序列并非由DNA编码。杂交分析表明，转移信使RNA基因以单拷贝形式存在。在整个细胞周期中，转移信使RNA都有相当高水平的积累；然而，在对数后期到稳定期，其水平会略有增加。这表明转移信使RNA不仅在细胞活跃分裂时发挥作用，在细胞生长停止时也有功能。