小鼠mSox13 cDNA的克隆与鉴定

Cloning and characterization of mouse mSox13 cDNA.

作者信息

Kido S, Hiraoka Y, Ogawa M, Sakai Y, Yoshimura Y, Aiso S

机构信息

Tokyo Electric Power Hospital, Japan.

出版信息

Gene. 1998 Feb 27;208(2):201-6. doi: 10.1016/s0378-1119(97)00667-7.

DOI:10.1016/s0378-1119(97)00667-7

PMID:9524265

Abstract

A novel SRY-related cDNA, mSox13, was isolated from a lambda phage library derived from mouse embryo. The cDNA encodes a protein of 595 amino acids containing the SRY-type high mobility group (HMG) box and a putative leucine zipper motif. A sequence comparison of mSox13 and other type-D SOX proteins shows that the leucine zipper and a neighboring glutamine-rich sequence stretch, which was named Q box, are well conserved among known type-D SOX proteins. The expression of mSox13 is restricted to the kidney and ovary. The electrophoretic mobility shift assay indicates that the recombinant mSox13 protein is capable of binding to the AACAAT sequence.

摘要

从源自小鼠胚胎的λ噬菌体文库中分离出一种新的与SRY相关的cDNA，即mSox13。该cDNA编码一种含有SRY型高迁移率族（HMG）盒和一个假定的亮氨酸拉链基序的595个氨基酸的蛋白质。mSox13与其他D型SOX蛋白的序列比较表明，亮氨酸拉链和一个相邻的富含谷氨酰胺的序列片段（称为Q盒）在已知的D型SOX蛋白中高度保守。mSox13的表达局限于肾脏和卵巢。电泳迁移率变动分析表明，重组mSox13蛋白能够与AACAAT序列结合。