小鼠SRY相关cDNA，即mSox7的分离与鉴定。

Isolation and characterization of a mouse SRY-related cDNA, mSox7.

作者信息

Taniguchi K, Hiraoka Y, Ogawa M, Sakai Y, Kido S, Aiso S

机构信息

Department of Anatomy, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.

出版信息

Biochim Biophys Acta. 1999 May 14;1445(2):225-31. doi: 10.1016/s0167-4781(99)00047-0.

DOI:10.1016/s0167-4781(99)00047-0

PMID:10320775

Abstract

SOX is a family of SRY-related genes, which encode transcriptional factors involved in development. In this study, we newly isolated and sequenced mouse cDNA clones for mSox7. The mSox7 gene encodes 380 amino acids containing an SRY-type HMG box. Genomic Southern analysis suggested that the mSox7 gene was a single-copy gene. Tissue specific expression of mSox7 was investigated by Northern analysis. The expression was restricted to the ovary and heart, and the size of the transcripts was estimated to be 3.6 knt. Electrophoretic mobility shift assay indicated that recombinant mSox7 polypeptide was capable of binding to a nucleotide sequence, AACAAT. Immunohistochemical study revealed that mSox7 protein was localized in oocytes in the mouse ovary.

摘要

SOX是一个与SRY相关的基因家族，其编码参与发育过程的转录因子。在本研究中，我们新分离并测序了小鼠mSox7的cDNA克隆。mSox7基因编码含SRY型HMG框的380个氨基酸。基因组Southern分析表明mSox7基因是单拷贝基因。通过Northern分析研究了mSox7的组织特异性表达。该表达仅限于卵巢和心脏，转录本大小估计为3.6千碱基。电泳迁移率变动分析表明重组mSox7多肽能够结合核苷酸序列AACAAT。免疫组织化学研究显示mSox7蛋白定位于小鼠卵巢的卵母细胞中。