Herbst H, Sauter M, Kühler-Obbarius C, Löning T, Mueller-Lantzsch N
Institut für Pathologie, Universitätskrankenhaus Eppendorf, Hamburg.
APMIS. 1998 Jan;106(1):216-20. doi: 10.1111/j.1699-0463.1998.tb01338.x.
Prompted by the observation of retroviral particle formation in teratocarcinoma cell lines and the consistent finding of antibodies against Gag and Env proteins encoded by human endogenous retrovirus (HERV)-K genomes in the sera of patients with classical seminoma, we studied ovarian and testicular germ cell tumours, their precursor lesions, dysgenetic gonads, and trophoblast lesions for expression of HERV-K sequences by in situ hybridization using radioactive and non-radioactive probes. HERV-K transcripts were detected in all testicular and ovarian germ cell tumours with the exception of teratomas and spermatocytic seminomas. HERV-K expression was also common to testicular carcinoma in situ as well as gonocytes of dysgenetic gonads. Among gestational trophoblastic lesions, HERV-K expression was regularly found in choriocarcinomas, but not in molar lesions. The patterns of HERV-K expression suggest a common molecular pathogenesis of most germ cell tumour entities and malignant gestational trophoblastic disease. They furthermore support the concept of carcinoma in situ as a precursor lesion common to most testicular germ cell neoplasms. The detection of HERV-K gene products in body fluids and tissues may aid diagnosis and monitoring of germ cell tumours and related lesions.
鉴于在畸胎瘤细胞系中观察到逆转录病毒颗粒形成，以及在经典精原细胞瘤患者血清中持续发现针对人类内源性逆转录病毒（HERV）-K基因组编码的Gag和Env蛋白的抗体，我们使用放射性和非放射性探针，通过原位杂交研究了卵巢和睾丸生殖细胞肿瘤、它们的前驱病变、发育异常性腺及滋养层病变中HERV-K序列的表达情况。除了畸胎瘤和精母细胞性精原细胞瘤外，在所有睾丸和卵巢生殖细胞肿瘤中均检测到了HERV-K转录本。HERV-K表达在睾丸原位癌以及发育异常性腺的生殖母细胞中也很常见。在妊娠滋养层病变中，绒毛膜癌中经常发现HERV-K表达，但在葡萄胎病变中未发现。HERV-K的表达模式提示大多数生殖细胞肿瘤实体和恶性妊娠滋养层疾病存在共同的分子发病机制。它们还进一步支持原位癌是大多数睾丸生殖细胞肿瘤共同的前驱病变这一概念。在体液和组织中检测HERV-K基因产物可能有助于生殖细胞肿瘤及相关病变的诊断和监测。
