Atkins K, Berry J E, Zhang W Z, Harris J F, Chambers A F, Simpson R U, Somerman M J
Department of Periodontics/Prevention/Geriatrics, University of Michigan, Ann Arbor 48109-1078, USA.
J Cell Physiol. 1998 May;175(2):229-37. doi: 10.1002/(SICI)1097-4652(199805)175:2<229::AID-JCP13>3.0.CO;2-3.
Promyelocytic leukemia HL-60 cells promoted by PMA to differentiate along the monocyte pathway adhere to tissue culture plates. To explore the regulation of adhesion molecules in cells promoted to differentiate, the expression and secretion of osteopontin (OPN) and expression of associated cell surface receptors, CD44 and integrin subunits alpha(v), beta3, beta1, were examined. Results were as follows: 1) PMA induced OPN mRNA and OPN secretion into media; 2) untreated cells expressed beta1 and CD44 mRNA, and PMA induced alpha(v), and beta3 mRNA and increased beta1 and CD44 mRNA expression; 3) PMA increased levels of alpha(v), beta3, beta1 and CD44 protein on the cell surface; and 4) retinoic acid, which promotes granulocytic differentiation of HL-60 cells, did not affect OPN, alpha(v), beta3, beta1, or CD44 mRNA or protein expression. These data suggest that induction of OPN and associated receptors may play a role during monocytic differentiation of HL-60 cells.
经佛波酯（PMA）诱导沿单核细胞途径分化的早幼粒细胞白血病HL-60细胞会黏附于组织培养板。为了探究促进分化的细胞中黏附分子的调控机制，研究人员检测了骨桥蛋白（OPN）的表达与分泌，以及相关细胞表面受体CD44和整合素亚基α(v)、β3、β1的表达。结果如下：1）PMA诱导OPN mRNA表达并促使OPN分泌到培养基中；2）未处理的细胞表达β1和CD44 mRNA，PMA诱导α(v)和β3 mRNA表达，并增加β1和CD44 mRNA的表达；3）PMA增加细胞表面α(v)、β3、β1和CD44蛋白的水平；4）促进HL-60细胞向粒细胞分化的视黄酸不影响OPN、α(v)、β3、β1或CD44的mRNA或蛋白表达。这些数据表明，OPN及相关受体的诱导可能在HL-60细胞的单核细胞分化过程中发挥作用。
