Golovkina T V, Dzuris J, van den Hoogen B, Jaffe A B, Wright P C, Cofer S M, Ross S R
Department of Microbiology/Cancer Center, University of Pennsylvania, Philadelphia 19104-6142, USA.
J Virol. 1998 Apr;72(4):3066-71. doi: 10.1128/JVI.72.4.3066-3071.1998.
Mouse mammary tumor virus (MMTV) infects a number of different cell types, including mammary gland and lymphoid cells, in vivo. To identify the cellular receptor for this virus, a mouse cDNA expression library was transfected into Cos-7 monkey kidney cells, and those transfected cells able to bind virus were selected by using antibody against the virus's cell surface envelope protein, gp52. One clone isolated from a library prepared from newborn thymus RNA, called MTVR, was able to confer virus binding to both monkey and human cells; this binding was blocked by anti-MTVR antibody. Moreover, transfection of MTVR into CV1 cells rendered them susceptible to infection by a murine leukemia virus-based retrovirus vector pseudotyped with the MMTV envelope protein. An epitope-tagged MTVR cofractionated with cellular membranes. Coimmunoprecipitation of the MMTV envelope protein and a MTVR-rabbit Fc fusion protein showed that these two proteins bound to each other. The MTVR sequence clone is unique, shows no homology to known membrane proteins, and is transcribed in many tissues.
小鼠乳腺肿瘤病毒（MMTV）在体内可感染多种不同类型的细胞，包括乳腺细胞和淋巴细胞。为了鉴定该病毒的细胞受体，将小鼠cDNA表达文库转染到Cos-7猴肾细胞中，然后使用针对病毒细胞表面包膜蛋白gp52的抗体筛选出能够结合病毒的转染细胞。从新生胸腺RNA制备的文库中分离出的一个克隆，称为MTVR，能够使病毒与猴细胞和人细胞都发生结合；这种结合被抗MTVR抗体阻断。此外，将MTVR转染到CV1细胞中，使它们易受以MMTV包膜蛋白为假型的基于鼠白血病病毒的逆转录病毒载体的感染。一个表位标记的MTVR与细胞膜共分离。MMTV包膜蛋白与MTVR-兔Fc融合蛋白的共免疫沉淀表明这两种蛋白相互结合。MTVR序列克隆是独特的，与已知膜蛋白无同源性，且在许多组织中都有转录。
